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Method
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Method |
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Methylation analysis is an often employed method in polysaccharides linkage analysis. This approach for determining linkage positions was developed in the late 1960s and early 1970s. The principle of this method is to introduce on each free hydroxyl group of the native oligosaccharide a stable substituent (methyl group). The glycosidic linkages are then cleaved, producing individual monosaccharide residues with new free hydroxyl groups that appear at the positions that were previously involved in a linkage. Today, the monosaccharides are derivatized to produce volatile molecules amenable to GLC-MS analysis. Several studies were performed on this technique and different methodologies are available in the literature (see references).
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1) S. Hakomori, J. Biochem. (Tokyo) 1964, 55, 205
2) P. A. Sandford, and H. E. Conrad, Biochemistry 1966, 5, 1508
3) I. Ciucanu, and F. Kerek, Carbohydr. Res. 1984, 131, 209
4) J. M. Kamerling and J. F. G. Vliegenthart, Carbohydrates. In: Clinical Biochemistry - Principles, Methods, Applications. 1989 Vol. 1, Ed. A. M. Lawson. De Gruyter, Berlin. P.p 176-263
5) C. Laine, T. Tamminen, A. Vikkuka, and T. Vuorinen, Holzfoschung 2002, 56, 607
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News
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Le 01/07/2010
EURaSYP Position Paper on the Regulatory Situation of Organic Yeast Extracts in the EU
Introduction:
By the 1st of January 2009, Organic Regulation (EC) No 2092/91 has been repealed and replaced by Council...
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FAQ
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Are the Specialty Yeast Products marketed by the EURaSYP members produced from GMOs (Genetically Modified Micro-organisms) ?
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